We provide services and reagents, at competitive prices,
for molecular biology and microbiology research. Our services
are available to all Tufts scientists.
The
core prepares competent cells by one of two methods: electrocompetent cells
(cells successively washed in glycerol that are ready to
receive DNA by electroporation) or CaCl2 competent cells
(cells that are primed by treatment with CaCl2 to
pick up DNA following heat shock). The core has an extensive
collection of host cells, providing investigators with
a wide range of options for transformation. The following
are most frequently requested:
| BL21 |
BL21DE3 |
| BL21DE3lysS |
BL21DE3lysE |
| C600 |
DH5a |
| CJ236 |
XL1Blue |
| MC1000 |
MC1061 |
| MC4100 |
HB101 |
| JM105 |
JM107 |
| JM109 |
JM110 |
| S17-λ1pir |
DH10b |
| DH5aλpir |
SM10λpir |
| CJ236/p3 |
fg2p3 |
| MC1061/p3 |
BW313/p3 |
We will
also prepare competent cells of strains provided by the
investigator. Cells are made in batches of ~60 aliquots
of 50microliters each. The transformation
efficiency of each batch is measured before it is released.
Electrocompetent cells must have an efficiency >107 transformants
per micrograms DNA; CaCl2 competent
cells, >105.
Ordering:
Batches of standard strains may be ordered simply by contacting the laboratory
with a request. Preparation of cells from custom strains are ordered by
delivery of the plate to the core lab. Plates must be clearly labeled with
the name of the PI, cost center to be charged for the procedure, and the
person to contact when the plasmid is ready. Turnaround time is ≤ two
weeks.
Services