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FACILITY INFORMATION
Facility Director
Anne V. Kane, M.D.
akane@tufts-nemc.org
Technical Staff
Lynn Gorczyca
Location
Tupper Building, Room 822 15 Kneeland Street
Tufts-New England Medical
Center
Mailing Address
750 Washington Street, Tufts-NEMC
Boston, MA 02111
Telephone
617-636-7611 |
We provide services and reagents, at competitive prices,
for molecular biology and microbiology research. Our services
are available to all Tufts scientists.
The core
maintains a liquid chromatography setup including a peristaltic
pump, UV monitor and recorder, fraction collector, gradient
maker, and a variety of gels (molecular sieve, ion exchange,
and chromatofocussing). The core also has an ion exchange
column for use with an FLCP setup to which the core has
access. Fusion proteins recently prepared include 6-histidine/NiNTA,
maltose binding protein (MBP), and glutathione S transferase.
Custom purification must be discussed with the core laboratory
facility director. Although the core staff often consults
in purification using multistep methods and can conduct
such purifications if needed, we expect the actual development
of the protocol to be done by the investigator.
As a service
to investigators, the core has purified taq polymerase
and dispenses it in 100µl aliquots. Shiga toxins are also available
to intestinal microbiologists.
Services
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